Lab 5 enzymes answers. This lab will help you practice the following skills: 1.

Lab 5 enzymes answers 1 What are enzymes? 2 What do enzymes do? 3 What is amylase? 4 What will act as the indicator in these experiments? a What will the indicator show? b What color BIOL 101: GENERAL BIOLOGY NAME: Semester: Lab 2: Enzymes (1 hr. This enzyme was used as it optimally active at alkaline pH environments and would allow the colorless pNPP to turn a yellow color, which helps us Essentials of Biology Laboratory Manual, Seventh Edition 45 LLABORATORY 5 How Enzymes Function Learning Outcomes Introduction Describe how an enzyme functions. Whether an enzyme is able to catalyze a specific biochemical reaction depends on its unique amino acid sequence and Selected Answer: c. Laboratory 5: How Enzymes Function. 5ml microcentrifuges labeled 0, 25, 40, 60, and 80 degrees Celsius were filled withed 10 drops of skim milk and 10 drops 3. 5 pt); b) Hypothermia (0. Enzymes are essential protein molecules that help sustain life in living organisms including (plants, animals, bacteria). We would like to show you a description here but the site won’t allow us. Question 2 1 out of 1 points The Lab 5: Enzymes, Diffusion, and Osmosis PART 1: ACTIVITY OF ENZYMES - CATALASE ACTIVITY 1A and 1B: Normal Catalase Reactions and Catalase Containing Tissues 1. Consists of acidic, neutral, and basic pH. They are biochemical catalysts meaning they lower the activation energy needed for a biochemical reaction to occur. What is the function of an enzyme. Grade - 5 - Final - Revision - Topics - 1,2,3 Science. Optimum pH is required to maintain the active Answer the following questions about enzymes and proteins. Substrates bind to the active site of a specific enzyme. What is Step 1? pour Biology Restriction Enzyme Lab Answers 5 answer explanations Tips and strategies for scoring higher from expert AP teachers and students who scored a perfect 5 on the exam End-of-chapter quizzes Targeted review of the most up-to-date content and disrupt bonds holding the enzyme in its precise shape). Lab 5: Amylase Enzyme Activity and Action of Inhibitors Overview Janelle S. Group of answer choices Conversion costs Costs that increase in direct relation to sales volume. Flashcards; Learn; Test; Match; Q-Chat; The location where the enzyme and substrate form an enzyme-substrate complex. Pre-Laboratory Question 1 - "? Lab 5 Enzyme Activity Post-lab worksheet For the post-lab worksheet, you should type your answers on a separate sheet. Apart from enzymes catalyzing biological processes in one’s own Lab 5 Review. Instructions: Answer the questions below based on Experiments 2 and 3. Explore the wonders of biology. Be specific with your explanation. • • End of preview Study with Quizlet and memorise flashcards containing terms like What's the overall purpose of this virtual lab?, What are the four basic steps involved in this bacterial identification lab?, What is 16s rDNA and how is it used to identify species of bacteria? and others. a protein that acts as a catalyst to speed up reactions by making them more energetically favorable. Flashcards; Learn; Test; Match; Created by. 2) The effect of temperature on enzyme activity in humans and thermophilic bacteria. Lab Lab05 Enzymes F24 (Answer Key). humans produce an enzyme (lysozyme) that attacks the open peptidoglycan layer - gram + are also more susceptible to beta-lactam antibiotics like penicillin Biology questions and answers; Lab Report 5 ENZYME ACTION Answer the following questions on Enzyme action in your own words: 1. An enzyme reduces the activation energy d. They are. Lab Goals: 1. 5. Enzyme activity at different pH levels Enzyme Activity (A/s) pH Average SD Acidic -1-04 2- Neutral 3-04 2- Basic 4-05 4- Table one: Average enzyme activity (A/s) for different pH levels along with the standard deviation. Lab 5 - Bacteria Identification Virtual Lab- solved. 1 / 98. The enzymes reacted similarly in each trial and the time it Study with Quizlet and memorize flashcards containing terms like What is the function of an enzyme, What is a substrate, What happens to the enzyme and the substrate during a chemical reaction and more. The virtual lab is interactive and goes through the step-by-step process of DNA fingerprinting 1. Substrates. INTRODUCTION Of all the elements and compounds that help make life possible, none ranks higher in importance than the group of molecules we call enzymes. Student Name: BSC 1010L Lab 5: Enzymes Lab Report (45 points) Directions: Use this lab report to record your lab results and answer the questions AS you proceed through the simulations for this lab module. 8/5/2020. 5 ml of the unstable enzyme with 9. Identify the role of hydrogen peroxide, oxygen, and catalase in this enzyme reaction 3. each micrograph contains a section of a nucleus and some cytoplasm. The molecules that bind to enzymes. a) Fever (0. txt) or read online for free. What is denaturation? a. We will use other enzymes which is why we have to inactivate the proteolytic enzymes. lock and key 7. You will compare the effect of lactase in the breakdown of lactose into glucose and galactose and the breakdown of sucrose into glucose and fructose. 5 pt). Changes in E shape at active site will impair or destroy its substrate-binding ability. denature 9. (1 point) Summarize the results you obtained for Study with Quizlet and memorize flashcards containing terms like In the peroxidase reaction, the substrate guaiacol is _____ when reduced and the product is _____ when oxidized: Active. starch over time : This lab will help you practice the following skills 1. d. What was the enzyme that was used and why? a. PDF. catalyst ___6_ 4. Oso Flaco Lake. Share. About us. e main enzyme for this lab, peroxidase, is found in many different forms, with optimum pHs ranging from 4 to 11 depending on the source and optimum temperatures varying from 10 to 70°C. Marley Tyler. enzyme. As most other biological components, enzymes require specific physiological conditions in order to maximize their functional performance as catalysts. PLAN UP TO 3 HOURS FOR THIS LAB – if you want to split it up it would be convenient to do Activities 1-3 one day (approximately 1 hour) and Activities 4and 5 on a second day (approximately 1 hour each). How could you test to see if an enzyme was completely saturated during an experiment? Not saturated: When increasing the substrate concentration and the rate of reaction increases Saturated: Rate of the reaction does not 5MH Enzymes Lab Report 45. As stated in the lab manual, for this exercise you will be repeating steps from exercise 5 to measure the enzyme activity while being inhibited by a Proteins that catalyze reactions are known as enzymes. Scientific Method examples answer key; Conversions and Dilutions Practice Problems; Lab 2 - Flow sheet Enzymes lab. What happens to enzymes exposed to very high temperatures? IV. 0 (1 review) Flashcards; Learn; Test; Match; Get a hint. 0 (4 reviews) Flashcards; Learn; Test; Match; Get a hint. Variable Laboratory 5: Transforming Bacteria with the Ligation Products B–56 observations, results, and answers to questions in a science notebook, in pen. Lesson 2: Virtual Lab Observing Bacteria, Protist, and Fungi. Microbiology Lab 5. Use the permanent marker to label 4 plastic cups as follows: 5% sucrose, milk, Which enzyme did we use in lab 6 that is a synthesis enzyme? A) catalase B) phosphorylase C) amylase D) lysozyme E) B and C. Each answer will only be used once. protein ___2_ 3. 2021/2022. Part B = Lab Part C = Lab Part D =13- instructions read: electron micropgraphs represent extremely thin slices of cells. The Effects of Temperature on Enzyme Activity MATERIALS AND METHODS Five 1. en Change Language. It begins in the cytosol with glycolysis and is completed within the mitochondria. 5 Catalase Activity Identify the substrate, enzyme, and product in the reaction studied today. 4) Variation in copies of the AMY1 Digestive enzymes are used in chemical digestion to break down large insoluble molecules to smaller soluble molecules. Ryan Name(s): An enzyme binds to a substrate at the _____. Enzymes are organic catalysts ie. View Lab - Lab 5_Enzymes Diffusion and Osmosis Answer Sheet. Catalase is an enzyme found in most living things. The _____ model of enzyme action states that enzymes change shape slightly when they bind to their substrate. destroys chemical reactions. Enhanced Document Preview: LAB 5 Enzyme Activity Big Idea 4: Interactions. Formal Lab Report Experiment 5 Enzymes: The objective of this experiment was to examine the effects of pH, enzyme concentration, and temperature on the catalytic rate of alkaline phosphatase (ALP) Anna Chen BIOL 1500 Victor Predict whether enzymes can be reused, or not *** Use saved test tubes 1 (no enzyme) and 2 (concentrated enzyme) from Exercise 2*** Notes on Answer Sheet (pages 101-105): A. stops chemical reactions. jillian_boudoin_lee. Farida M. Enzymes can participate in reactions with all the organic molecules of life (carbohydrates, proteins, lipids, and nucleic acids). 5. BIO 1134 - Biology I Lab 5: Enzymes, Diffusion, and Osmosis R. To do so, the sample is placed in a heated water bath at 100 degrees Celsius. Pipette 4ml of the substrate that is appropriate into each labeled tube. Put 4. slows chemical reactions. Biology 1140L Spring 2020 Lab Report 2: Enzymes: Answer Sheet Please type your answers into this answer sheet, and submit only the completed answer sheet to your TA. All the chemical reactions that occur in a cell. pptx. speeds up chemical reactions. the result of a chemical reaction d. Because of enzyme activity, cells can carry out complex chemical activities at relatively low temperatures. inhibitor __4__ 6. 1 / 7. active site __1__ 1. You will Biology questions and answers; LAB 5 ENZYMES Introduction: Chemical reactions involve the breaking and formation of bonds. Biology questions and answers; Name: Date: POST-LAB QUESTIONS - Enzymes-Lab #5 1. Lab 1 5: Field Trip. The enzyme that was used was alkaline phosphatase. Exercise Number 5 Enzyme Kinetics. Lab # 5 Enzyme Activity Objectives: After completion of the laboratory activities, students will be able to: 1. Part A 1. Course: General Biology II: (Majors) SUN# BIO1182 (BIO182IN) 9 View Lab 3 answer sheet. In Study with Quizlet and memorize flashcards containing terms like enzyme, substrate, activation energy and more. Answers: a. Enzyme Lab Write-up; Protein Lab Write Up; Lab 1: Amino Acids Write-up WITH ANSWERS; Protein Lab Write-up WITH ANSWERS F2021; Key Points for Lab 2 Protein Lab; Related documents. Enzymes are not used up in the chemical reaction c. 09/21/ Pre-Lab Questions. pdf. (For scientific purposes, it is important to keep a record—even of your mistakes. Protein molecules that are catalysts that dramatically speed up biochemical reactions. Cascade Lab 5: Enzymes & Osmosis 6 Assay Table: You will use the following assays to determine See an expert-written answer! We have an expert-written solution to this problem! An enzyme reduces the energy necessary to start a chemical reaction. all enzymes fit into this class of macromolecules c. The enzyme releases the products. Some people experience gas and discomfort ##### after eating vegetables, grains, beans, and BIOL 112 Lab 5 - Lab report on Harvard Museum of Natural History Exercise 1 - Enzyme Specificity In this exercise, you will determine the ability of lactase to be specific and to catabolize. What do enzymes do? 3. BIOC However, soil pH usually is between 5 and 6. pdf from BIO 1134 at Mississippi Gulf Coast Community College. Use the following terms to explain enzyme reactions: enzyme, substrate, product, rate, active site, optimum and denature 2. 3 pages. 3) Why enzyme activity stops at different temperatures in humans and bacteria. Flashcards; Learn; Test; Match; Q-Chat; Created by. They often work in conjunction with vitamins and minerals to act as catalysts, accelerating chemical reactions that would otherwise occur too slowly within cells. substrate ___7_ 2. Enzymes. In this lab, we will run computer simulations to examine the Lab 5- enzymes. Lab # 11: Enzyme Activity. Part A = 1-12; match the cellular components with the descriptions. Gain a better understanding of enzymes and some conditions that affect enzyme activity and the rate of Lab 5: Investigating Enzyme Activity (15 points) Submission: To submit, you can directly draw/type your answer on this document or print this document, complete all lab activities and answer the review questions, and 5 Lab #3: Enzyme Activity Lab Report (10pts) Answer the questions below, thinking through the specific concepts that generated the results in your experiment. As could be predicted, the purified enzyme from a common soil fungus has a pH optimum of 5. 100% (8) 2021/2022 100% (8) Save. Specificity of Enzyme Use last page (pg. a molecule that prevents an enzyme from working e. Lab # – Part 2: Enzyme Data Analysis. lactase. Enzyme activity was highest at a high enzyme concentration level. Biology 1140L Fall 2019 Lab Report 2: Enzymes: Answer Sheet Please type your answers into this answer sheet, and submit only the completed answer sheet to your TA. 5 ml of BSA 2. Metabolism. How do you determine the amount of enzyme present in a sample? In a sample solution, the amount of enzyme can be determined in an experimental procedure called enzyme assay by obtaining the enzyme activity of the solution wherein the amount of product formed or substrate consumed during an enzyme-catalyzed Step 6: Draw Conclusions In a complete sentence, answer your experimental question by summarizing the data. they speed rates of chemical reaction and are not themselves Answer) 1. The functions that a cell can perform are largely dependent on the enzymes that the cell produces. The boxes show some large insoluble molecules, some digestive enzymes and some smaller soluble molecules that Lab 5 - Enzyme Quiz Answers. Practice experimental design. Final Exam w 2011 A answers II amino acid write up -- Lab 02: protein write up -- Lab 03: enzyme kinetics write up -- Lab 04: lipid lab write up -- Lab 05: carbohydrate write up --19 pages. The _____ model of enzyme action states that enzymes are specific to one substrate. ) enzymes, a whole new era of genetic engineering was launched. JusticeComputerYak3. In this lab you conducted experiments with 3 different enzymes that each participated with one of the organic molecules of life. Flashcards; Learn; Test; Match; Q-Chat; Get a hint. In this lab we will make up mock cells using a material called dialysis tubing. What are two factors that can affect an enzymes ability to catalyze a reaction? 3. Lab 4: Quantitative Analysis of Enzyme Activity Lab Worksheet. I. BIO. 4- Enzymes Virtual Lab. harmless Colorless. Oso Flaco Lake Answer Key. EFFECT OF pH ON ENZYME ACTIVITY 1. What are enzymes? I 2. Practice and apply hypothesis testing. , Which of the following are true of an enzyme-catalyzed reaction? a. As could be predicted, the puri ed enzyme from a common soil fungus has a pH optimum of 5. This lab will help you practice the following skills: 1. Describe three factors that can lead to the denaturing of enzymes. 1. Who are the suspects? 7 sisters. An enzyme speeds up the conversion of substrate to product b. _____________________; b. In this lab, you will study an enzyme that is found in the cells of many living tissues. SUBSTRATES. Purpose: The purpose of this lab is to: 1) perform a colorimetric assay to determine the amount of starch in a solution, and to 2) calculate the rate of an enzyme reaction by analyzing the breakdown of . for each exam of the series. Lab 5 Cell Respiration Introduction Cellular respiration is a series of enzyme-mediated reactions that release the energy from carbohydrates. Stabilize unstable enzyme by mixing 0. Table 3: Class Averages for Enzyme Temperature Standard Deviation Calculations Enzyme Activity (U) Enzyme Temperature Average SD 4C 23C 32C 60C 3. a molecule The document summarizes a lab quiz on enzymes. Lab 2 Enzyme Catalysis 228 Lab 3 Mitosis and Meiosis 229 Lab 4 Plant Pigments and Photosynthesis 230 Lab 5 ANSWERS AND EXPLANATIONS TO THE CHAPTER QUIZZES 235 17 THE PRINCETON REVIEW AP BIOLOGY PRACTICE TEST 1 249 18 2020-08-11 Make sure you’re studying with the most up-to-date prep materials! Study with Quizlet and memorize flashcards containing terms like In chemical reactions, what are the molecules that undergo a change called?, In chemical reactions, reactant molecules undergo change and result in the ______________, What are enzymes? and more. 5 Effect of 1. Enzyme Catalysis Gabryele Koykka 10/11/2020 1 Data Activity 1 Data Table 1 0% 25% 50% 75% 100% Depth of List three conditions that would alter the activity of an enzyme. Most enzymes are proteins. b. Joanna 1 LAB 6A MANUAL: ENZYMES: BIOLOGICAL CATALYSTS OF LIFE Instructions: Provide answers to five questions after the first experiment and two questions after the second experiment. Enzyme Action Answer the following questions. Answer to Questions Q1. Take a look around your house and identify household products that this set is graded and should be correct. How do abiotic or biotic factors influence the rates of enzymatic reactions? Please be sure you have read the student intro packet before you leave this lab. Your instructor may require this page for you to enter the lab. Every time a substrate is Lab 5: Characterizing the Enzyme Alkaline Phosphatase. Why are enzymes essential for life? 2. If the shape of the active site changed on an enzyme, would the enzyme still be able to catalyze the same substrate? Why or why not?. a enzyme that digests milk. 2. Lab15 Ocean Beach Park F24. What crime was committed? lollipop was licked. Study with Quizlet and memorize flashcards containing terms like Enzymes speed up the rate of a chemical reaction. Lab 5 Enzyme Activity. usually is between 5 and 6. Procedure 1. For every pH prepare a blank by adding 0. Complete the Pre-Lab assignment before coming to lab. In general, what do unfavorable environmental conditions do the speed of an enzymatic reaction? Answer the following questions in the spaces provided. Explore quizzes and practice tests created by teachers and students or create one from your course material. 100% (2) Lab 5 - Enzyme Quiz Full Lab Report Enzymes. This enzyme catalyzes the Biology questions and answers; LAB 5 ENZYMES Introduction: Chemical reactions involve the breaking and formation of bonds. docx. The quiz contains questions about: 1) Which mixtures would cause a reaction catalyzed by the enzyme manganese dioxide. 18 October, 2021 Lab 5: Properties of Enzymes Post-Lab Assignment Part I: Lab Notebook Questions 1. a portion of an enzyme to which a substrate can attach b. does catalase. What are the 2 main differential stains for bacteria? Gram stain Acid fast stain. they speed rates of chemical reaction and are not themselves consumed in the reaction. Temperature, pH, and concentration can alter the activity of an enzyme. The Biology questions and answers; Lab 5: Enzyme Kinetics Worksheet Name: Part 1: Questionnaire Commercial + Wheat Germ Michaelis-Menten Plot 1- What is an enzyme? 2- What is a substrate? 0. (1 point) Use your Experiment 1 results to express the activity of beta-galactosidase as a reaction rate. Increase the free energy of a Biological Macromolecules and Enzymes. reddish brown Colorless. BSC 108. Presentation Slides Lab 6: Cellular Respiration. As a result, enzymes denature at extremes of pH and high salt concentrations. Label 5 test tubes (glucose, sucrose, lactose, corn starch, and water) 2. Lab15 Oso Flaco Lake F24. What key terms can be used here? Answer • Enzymes are proteins that catalyze metabolic reactions. Rachel Nguyen TA: Bennett Perry Lab section E 7 October 2021. Eva_Behr Teacher. Close suggestions Search Search. Mahaz N. Enzyme Activity Objective: The purpose of this laboratory exercise is to demonstrate enzymatic reactions and how variables like Study with Quizlet and memorize flashcards containing terms like What does an enzyme do?, Enzyme, Substrate and more. The amount of oxygen generated will be measured and used to calculate the rate of the Substrate Enzyme Enzyme-Substrate Product Product Enzyme 1. induced fit 8. The name of the enzyme is catalase; it speeds up a reaction which breaks down hydrogen peroxide, a toxic chemical, into two harmless substances → water and oxygen. They are Enhanced Document Preview: Biological Macromolecules and Enzymes Answer Key Lab 3 Biological Macromolecules & Enzymes *Read and complete LAB 3 Biological Macromolecules & Enzymes guide. Why does enzyme specificity promote enzyme activity? The active site of an enzyme has a shape that is complementary to the shape of its substrate. 5) of this ‘Notes’ Enzyme Guided Notes Answer Key - Free download as PDF File (. 5) Answer the question: Is catalase reusable? Part B - What Tissues Contain Catalase. ##### 9. Lab #5. Question: What is an enzyme and how do they catalyze a reaction. : Our hypothesis was not supported because the yeast spheres remained floating in the hot, cold and room temperature water. Purpose: The purpose of this lab is to: 1) perform a colorimetric assay to determine the amount of starch in a solution, and to 2) calculate the rate of an enzyme reaction by analyzing the breakdown of starch over time. Skip to document. Lab05 Enzymes F23. Access easy-to-understand explanations and practical examples on key biology topics, from cells Lab 5 Quiz Practice Questions and Answers; Pdf - quiz for biochemistry. 5 Effect of Temperature on Enzyme Activity Predict the effect of temperature on an enzymatic reaction. 6. Open navigation menu. Again the pH at which an enzyme works best is called its optimal pH. When testing the temperature of catalase in the enzyme lab, why did the higher temperature affect the catalase activity? A. BIOC 2580 - Lab 1 - lab 1; Lab 4 - lab 4; Lab 5 - revised - lab 5; Lab 4 Quiz Answers 1. 1 / 16. compare the organelles shown in these micrographs with organelles of the Lab 2 Enzyme Catalysis Introduction: Enzymes are proteins produced by living cells. docx from BIO 103 at Rowan College, Burlington County. 5 ml of BSA and add an experimental solution by 7 Lab 5- Enzyme Lab Fall2024; BIOL1108L Biodiversity Worksheet online; Preview text. 2 – Enzyme Catalysis Introduction: This lab will observe the conversion of hydrogen peroxide to water and oxygen gas by the enzyme catalysis. Write the chemical reaction that has taken place in your positive control using this format: Enzyme + Substrate (enzyme-substrate complex) Enzyme + Product 7. lab answers lab how enzymes function questions what is the purpose of an enzyme? main purpose of an enzyme is to help speed up metabolism. Total views 13. Creating a DNA Fingerprint. Due Date: The beginning of your scheduled lab section during the This and salt concentration also affect enzymatic structures. Describe the effect of temperature, pH, enzyme concentration, substrate concentration, and presence of cofactors and inhibitors on enzyme Quiz yourself with questions and answers for BIO LAB PRACTICE EXAM (Lab 5 - Enzymes), so you can be ready for test day. 5 ml of PNPP at pH 4,6,8,11 and 13 in two test tubes for each pH 3. Scientists now To answer this question, describe what happens to human enzymes in each situation. Note on testing for glucose with the test strips: The test The average enzyme activity was recorded at low, medium, and high levels of enzyme concentration. INTRODUCTION. c. . Lab15 Oso Flaco Lake F24 (Answer Key). Abstract Enzymes are multi-structured proteins that behave and function as biological catalysts. The active site of an enzyme changes shape, breaking the bond within the substrate. 3. How would denaturing an enzyme affect its activity? (4 marks = 3 for factors, 1 for explanation) Factors: Lab 5: Characterizing the enzyme alpha amylase. About Quizlet; How Quizlet works; Careers Biology questions and answers; PRE-LAB QUESTIONS-Enzymes-Lab #5 1. Enzymes – Catalase Function Lab 6: Enzymes – Catalase Function OBJECTIVES. 1 / 10. Solved: lab 5 enzymes name: section: pre-lab questions-enzLab enzyme graph virtual Lab 5: enzymes and reactionsEnzymes lab solved experiment questions test pre could answer problem been has answers. BIO 101. ) – 20 Points PRE-LAB QUESTIONS (3 POINTS/1 POINT EACH) 1. Make sure to state whether your data supports your hypothesis or not. Inactive Toxic . Click or tap here to enter text. See an expert-written answer! The active site of an enzyme brings together the substrates of a reaction. 2. 4- 3- What's the name of the enzyme we are using in this lab? What's its function? 4- In this lab we are using an artificial substrate. blue, Enzymes like peroxidase are important biological catalysts because they: A. Define catalyst, enzyme, enzyme-substrate complex, substrate, product, active site, denaturation, cofactor, and inhibitor. The name of the enzyme is catalase; it speeds up a reaction which breaks down hydrogen peroxide, a toxic chemical, into 2 harmless substances--water and oxygen. What can cause an enzyme to denature? b. Answer Key. Using a spectrophotometer to determine the amount of a product in a solution 2. Record in Table 6-5 at Time 0 the relative color intensity of each tube immediately after adding the 1% Catechol. Biology questions and answers; Lab 5: Enzyme Kinetics Worksheet Name: Part 1: Questionnaire Commercial + Wheat Germ Michaelis-Menten Plot 1- What is an enzyme? 2- What is a substrate? 0. This further affects the enzyme-substrate binding. Your lab kit contains a sheet of labeled colored drawings of “Molecules Involved in ##### in your answer. Lab #5 Properties of Enzymes. Active site. Some of the hydrogen peroxide enzymes were denatured. Purpose: The purpose of this lab is to calculate the rate of an enzyme reaction by analyzing the accumulation of product over time. a. Today’s lab will focus on two different enzymes: catalase and catechol oxidase. Synthesis -- Answer the question: Is catalase reusable? CLAIM: EVIDENCE. The purpose is to determine the iodine number of a lipid sample, used to 7 Lab 6. Lab 5: Characterizing the enzyme alpha amylase. Will a Enzymes and Lactose Intolerance Lab. docx - Name the 4 ways that Pages 1. Save. Flashcards; Learn; Test; Match; Get a hint. Tidewater Community College. In addition, substrates and/or the enzyme's active site groups may become ionized. As with all most all proteins, the function of an enzyme is dependent on its structure. Using a spectrophotometer to determine the Bio-1 Dr. pdf), Text File (. (Step 6,Lab Manual, p60) Table 6-5 Effect of pH on Enzyme Activity Relative Color Intensity on a Scale of 0 to 5 Time (min) Tube IV Lab 5: Characterizing the enzyme alpha amylase. The main enzyme for this lab, peroxidase, is found in many different forms, with optimum pHs ranging from 4 to 11 depending on the source and optimum temperatures varying from 10 to 70°C. This is accomplished by decreasing the activation energy E for a chemical reaction In an enzyme 1 BIOL& 160 Lab Module 5: Lactase Enzyme Activity Introduction Enzymes are the cell's toolkit. Cellular Respiration can be summarized with the following equation: C6H12O6 + 6O2 → 6CO2 + After reading over the lab and the enzyme, diffusion and osmosis topics from your textbook, answer these questions to be turned in at the beginning of the lab! 1. The enzyme, now empty, is ready to be used again. very similar questions. 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